ABSTRACT
<p><b>OBJECTIVE</b>To study the curative effects of Xianling Qianggu koufuye (XLQG) on postmenopausal osteoporosis in ovariectomized female rats.</p><p><b>METHOD</b>Sixty female Sprague-dawley rats aged 12-months were used, 50 of them were ovariectomized and randomly divided into 5 groups: ovariectomized (OVX), OVX + Nylestriol, OVX + XLQG (high dose, middle dose, low dose), and the others were sham-operated group. Rats were treated with drugs starting at the 45 day after the operation for 90 days. Double in vivo fluorochrome labeling was administered to all rats. At the end-point of study, the blood was collected to detecte the contents of ALP and StrACP in serum, and the fourth lumar vertebra (LV4) and femur bone sections were cut and stained for bone histomorphometric analyses, biomechanical analyses and BMP analyses.</p><p><b>RESULT</b>XLQGKFY decreased greatly the StrACP content, increased BMP and bone stiffness, and improved the bone biomechanical property.</p><p><b>CONCLUSION</b>Xianling Qianggu koufuye has a curative effect on postmenopausal osteoporosis, which provides for clinical use.</p>
Subject(s)
Animals , Female , Humans , Rats , Acid Phosphatase , Blood , Administration, Oral , Alkaline Phosphatase , Blood , Bone Density , Cockroaches , Chemistry , Drug Combinations , Drugs, Chinese Herbal , Therapeutic Uses , Isoenzymes , Blood , Materia Medica , Therapeutic Uses , Medicine, Chinese Traditional , Osteoporosis , Blood , Drug Therapy , Osteoporosis, Postmenopausal , Blood , Drug Therapy , Ovariectomy , Plants, Medicinal , Chemistry , Random Allocation , Rats, Sprague-Dawley , Tartrate-Resistant Acid PhosphataseABSTRACT
<p><b>AIM</b>To study the chemical constituents of Ligularia dictyoneura.</p><p><b>METHODS</b>Various chromatographic techniques were used to separate and purify the compounds. Their spectral data (MS, IR, NMR) were measured for structure elucidation.</p><p><b>RESULTS</b>Four sesquiterpenes were isolated from Ligularia dictyoneura and their structures were identified as ligudicin A (1), ligudicin C (2), ligudicin D (3) and isopetasin (4).</p><p><b>CONCLUSION</b>Compound 1 is a new compound, compound 2 is a new natural product, and compounds 3 and 4 are obtained from Ligularia dictyoneura for the first time.</p>